Summary and explanation the mosquitoborne dengue viruses serotype 14 cause dengue fever, a severe flulike illness. The study of detection of dengue ns1 antigen and igm antibody. A commercial dengue ns1 antigencapture elisa was evaluated to demonstrate its potential application for early laboratory diagnosis of acute dengue virus infection. Introduction dengue, a flavivirus, is found in large areas of the tropics and sub. Paired sera from patients with dengue n20 and japanese encephalitis je, n10. Principle of the test the zikv detecttm igm capture elisa is an enzyme linked capture immunoassay for the detection of human igm antibodies targeting the zikv envelope glycoproteins. Evaluation of a new commercially available immunoglobulin m.
The focus diagnostics dengue virus igm capture dxselecttm is a qualitative assay for the detection of human serum igm. Pdf evaluation of the mrl diagnostics dengue fever virus. In summary, this study indicates that the inbios denv detect igm capture elisa is a reliable, rapid, sensitive, and specific serological test for detection of acute or recent dengue virus infections and thus can be employed by public health laboratories for rapid detection of dengue virus infections during dengue epidemics. Diagnosis of dengue by igm capture elisa with principle, procedure and result interpretation description of the test. Pdf comparison of panbio dengue igm elisa assay with. Indirect enzymelinked immunosorbent assay elisa for. Comparison of panbio dengue duo igm and igg capture elisa and. The study of detection of dengue ns1 antigen and igm.
Evaluation of commercially available anti dengue virus. Paired sera from patients with dengue n 20 and japanese encephalitis je, n 10, and single sera from patients with typhoid n 10, leptospirosis n 10 and scrub typhus n 10 were. During the first step, the igg antibodies present in the serum or plasma are captured by a human antiigg antibody coated on the microplate. Indirect igg versus igg capture elisa for dengue diagnosis. The reproducibilty of dengue igm and igg elisa was studied in serum and filter paper blood spots from vietnamese febrile patients. All samples tested shows dengue igm antibody more than 11 units. Antidengue serum igg is generally detectable at low titres at the end of the first week.
For the early detection of dengue virus ns1 antigen in human serum. Enzyme linked immunosorbent assay elisa for the determination of igm antibody to dengue virus in human serum or plasma. Assays for the serological diagnosis of dengue infections dengue igmcapture elisa panbio dengue iggindirect elisa panbio dengue ns1 antigen detection elisa panbio trainer. The results showed an agreement of 100 % with the quality assessment results. This method is reported to be as sensitive as the hemagglutination inhibition hi method 7. A comparative evaluation of dengue diagnostic tests based on. For the qualitative detection of igm antibodies to dengue virus in human serum. Dengue virus ns1 antigen was detected in 199 of 2 acute serum samples from patients with laboratory confirmation of acute dengue virus infection but none of the 354 healthy blood. Enzymelinked immunoassay for dengue virus igm and igg. Comparison of panbio dengue duo igm and igg capture elisa. South, suite 600, seattle, wa, usa, manufactures diagnostic tests for infectious diseases. Platelia dengue igg capture is an immunocapture assay designed to detect the specific high level of dengue igg.
Igmcaptureelisa of serum samples collected from filipino dengue patients october 2000 the southeast asian journal of tropical medicine and public health 3. This assay is this assay is specific for igm and uses a flavivirus group monoclonal horseradish peroxidase conjugate with tmb as substrate. Dengue, igm detection, niv igm capture elisa, panbio rapid immunochromatographic card test. Dengue antibodies igg elisa preparation, procedure, cost. Dengue virus is a singlestranded rna virus of about 50 nm in diameter belonging to the genus flavivirus. Evaluation of capture elisa and rapid immunochromatographic test for the determination of igm and igg antibodies produced during dengue infection. The elisa test kit provides a semiquantitative in vitro assay for human antibodies of the igm class against dengue viruses in serum or plasma.
Introduction the mosquitoborne dengue viruses serotype 14 cause dengue fever, a severe flulike illness. March 27, 2017 page 1 of 15 zikv detecttm igm capture elisa instructions for use for use under an emergency use authorization only purpose this document describes the use of an igm antibody capture enzyme linked immunosorbent assay macelisa for the. Dengue igm microlisa is designed for invitro qualitative detection of dengue igm antibodies in human serum or plasma and is used as a screening test for testing of collected blood samples suspected for dengue. Dengue igm and igg antibodies in 433 suspected dengue samples were determined using commercially available dengue antibody detection assays. Pdf igmcaptureelisa of serum samples collected from. We noted that rapid ict kits that we used for testing ns1ag and igm performed at par with elisa based tests. Brief communicationsserodiagnosis of dengue virus infection in. Eua zikv detecttm igm capture elisa insert part number 90021101 effective date. A number of commercial elisa for dengue diagnosis have recently become available, though direct comparison between these assays have not been published. The dengue virus igm elisa test system is an enzyme linked immunosorbent assay elisa for the detection of igm class antibodies to dengue virus in human serum or plasma.
Dengue virus igm elisa se120043 technical bulletin. Abcam s antidengue virus igm human in vitro elisa enzymelinked immunosorbent assay kit is designed for the accurate qualitative measurement of igm class antibodies against dengue virus in human serum and plasma. We base our values on how high over background values that the antibodies detect at. If i am pregnant and my test is positive for zika virus. Evaluation of a commercial dengue ns1 antigencapture elisa. Thane, mumbai for dengue ns1, igg and igm as per manufacturers instructions. The calbiotech dengue virus igg elisa kit is intended for the detection of igg antibody to dengue virus in human serum or plasma. For research use only, not for use in diagnostic procedures. A commercial dengue ns1 antigen capture elisa was evaluated to demonstrate its potential application for early laboratory diagnosis of acute dengue virus infection. A commercially available capture enzymelinked immunosorbent assay elisa for the detection of specific immunoglobulin m igm and igg antibodies produced during dengue infection panbio dengue. Significance of igg optical density ratios index value in single.
This assay doesnt detect low level of dengue igg ab present from past infection. Test name dengue igm capture pathozyme dengue m pathozyme dengue m capture dengue fever virus igm capture dxselect. Platelia dengue igg capture is an immunocapture assay performed in two steps. No special preparation is needed for dengue antibodies igg elisa.
Despite the lower detection rate for serum samples from patients with acute secondary dengue, this was still higher than the other dengue diagnostic methods. Igm capture elisa of serum samples collected from filipino dengue patients october 2000 the southeast asian journal of tropical medicine and public health 3. The venture technologies dengue igm and igg dot blot assays and the panbio dengue duo igm and igg capture elisa were compared. The diluted serum serum diluent contains sorbent to remove rheumatoid factor and human igg interference is added to wells coated with purified antigen.
Evaluation of a dipstick enzymelinked immunosorbent assay for detection of antibodies to dengue virus. Eden01g intended use the panbio dengue igg indirect elisa is for the qualitative detection of igg antibodies to dengue antigen serotypes 1, 2, 3 and 4 in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms and past exposure consistent with dengue fever. Introduction dengue, a flavivirus, is found in large areas of the tropics and subtropics. Elisa microwell method for the detection of igm antibodies is currently the most useful procedure for providing a specific serological diagnosis of dengue infection. Mac elisa test for the detection of dengue igm antibodies in human serumplasma dengue igm microlisa 1. Not for sale or distribution in the united states of. Zikv detecttm igm capture elisa instructions for use. Two easytouse commercial diagnostic assays, a dipstick enzymelinked immunosorbent assay elisa integrated diagnostics, baltimore, md. Ithas also been evaluated in some studies that, the ns1 antigencapture elisa gave a significantly higher detection rate in acute primary dengue than in acute secondary dengue. Inbios dengue igm elisa continues to prove fast and. Although most of the reported dengue cases in the united states are acquired by travelers or immigrants, autochthonous dengue fever outbreaks have occurred in brownsville, tx. The dengue virus igm elisa kit is intended for the detection of igm antibody to dengue virus in human serum or plasma. The company manufactures a full range of dengue products including elisa kits for dengue igm, igg, and ns1 and a rapid test for dengue ns1. Transmission is by mosquito, principally aedes aegypti and aedes albopictus.
Any of the routinely used tests like, viral nucleic acid detection by reverse transcription polymerase chain reaction rtpcr, dengue ns1 protein antigen or antidengue igmigg antibodies, can be used to diagnose a case of dengue, however, igm capture elisa has always been the choice due to its cost effectiveness, availability and ease of. Dengue ns1 antigen test helps in early detection of dengue. Dengue igm captureelisa kit is an invitro diagnostic test for screening for dengue igmantibodies, in primary and secondary infections caused by all 4 serotypes. Ei 266b9601 m dengue virus igm agcoated microplate wells 96 x 01 96 intended use. Recommended test is ns1 antigen by elisa in the first 5 days of fever. Accudiag test principle dengue ns1 antigen elisa kit. The disease is prevalent in third world tropical regions and spreading to subtropical developed countries including the united states. The panbio elisa product code dec400 was performed according to the manufacturers instructions.
Principle of the assay the qualitative immunoenzymatic determination of specific antibodies is based on the elisa enzymelinked immunosorbent assay technique. This test kit is intended of igm class antibodies against dengue virus in human serum or plasma. Evaluation of a new commercially available immunoglobulin. We compared two generations of panbio brisbane, australia commercial kits for ns1 antigen capture for early diagnosis of dengue. Its laboratory diagnosis, with special emphasis on. Dengue igm microlisa test is an enzyme immunoassay based on capture elisa. Comparison of two rapid diagnostic assays for detection of.
Platelia dengue igg capture is designed to detect ns1 specific igg useful to differentiate primary and secondary dengue virus infections 6, 7. Evaluation of rapid ict in comparison with macelisa in. After a first washing step to remove excess of sample, conjugate formed with. Specimens and controls are added to the microtiter wells and incubated. The igm elisa method is generally replacing other techniques for igm determination. Diagnosis of dengue by igm capture elisa with principle. Your doctor depending on your condition will give specific instructions. Of the 5 elisa kits evaluated, 3 focus, panbio, and standard showed strong agreement with reference stan. Evaluation of commercially available antidengue virus. This is because a single reactive antidengue igg capture elisa test gac elisa in the absence of igm antibodies or ns1 ag may denote current infection or. Dengue iggigm elisa kit parasitology antibody detection elisa kits. I use a capture elisa kit from panbio that works great in detecting human antidengue igg.
The presence of antidenv igm and igg antibodies were detected using the sd dengue igm and iggcapture elisa kits standard diagnosis inc. Two microtiter plates were supplied, one containing stabilized dengue 1 through dengue 4 antigen plate and the other containing either antihuman igm or antihuman igg bound to separate wells assay plate. Dengue igg igm elisa kit parasitology antibody detection elisa kits. Diluted patient serum serum diluent contains sorbent to remove rheumatoid factor and human igg interference is added to wells coated with purified antigen. Igm capture and igg capture elisas are the most common assays for this purpose. Furthermore, mabs d3 4248g, d1 606a12b9 and d1 69512c2h were used to develop a capture enzymelinked immunosorbent assay elisa for anti dengue igm detection in sera from patients with acute. All the samples were then subjected to dengue igmcapture elisa by inbios igm antibody capture macelisa, seattle, usa. Comparative analysis of rapid dengue testing and elisa for. Test procedures and interpretation of results were carried out in accordance with the manufacturers instructions. Dengue capture igm elisa capture igm, dengue capture igg elisa capture igg, and panbio dengue duo cassette kit dengue duo, in accordance with the manufacturers instructions inverness medical innovations. Apr 02, 2010 video describing the steps undertaken in performing elisa to detect igm antibodies against dengue in patient serum. This test will aid in the early diagnosis of dengue virus in human serum even prior to the presence of igm or igg antibodies. The zikv detecttm igm capture elisa tests for igm antibodies in human serum. Comparison of two generations of the panbio dengue.
The assay utilizes inactivated antigen for dengue virus types 1 4. The cookie settings on this website are set to allow all cookies to give you the very best experience. Antihuman igm antibodies are coated onto microtiter wells. Typical data obtained from human dengue virus igg elisa kit direct eia this data is generated from a recent batch of ek7045 human dengue virus igg elisa kit direct eia. A comparative evaluation of dengue diagnostic tests based. It is not necessarily the same data set used to generate the standard curve shown in the image of this product.
Evaluation of ns1 antigencapture elisa and igm antibody. This test may aid in the early diagnosis of dengue virus in human serum prior to the presence of igm or igg antibodies. Evaluation of nonstructural 1 antigen assays for the. The focus diagnostics dengue virus igm capture dxselecttm is intended for the detection of human serum igm antibodies to dv types 14. Early and accurate diagnosis of dengue is imperative for disease surveillance, which helps in the control of dengue in endemic countries. Panbio dengue igm capture elisawash buffer, sample diluent, antigen diluent buffer, dengue 14 antigen, hrp conjugate 1. Dengue virus igm capture dxselect focus diagnostics. Panbio dengue igm capture elisa wash buffer, sample diluent, antigen diluent buffer, dengue 14 antigen, hrp conjugate 1. Relevant identified uses of the substance or mixture and uses advised against not available not available safety data sheet sds according to regulation ec no 4532010 panbio dengue igm capture elisa doc. Video describing the steps undertaken in performing elisa to detect igm antibodies against dengue in patient serum. Enzyme immunoassay elisa for the qualitative determination of igm antibodies. Igm antibodies by rapid visual immunochromatographybased test ict ezdx dengue combo agab from advy chemicals pvt. A 96well plate has been precoated with dengue virus antigens to bind cognate antibodies.
Cross reactivity is seen in the flavivirus group between dengue virus, murray valley encephalitis. The panbio dengue igm capture elisa should be used in conjunction with other dengue serology. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. In this study, we evaluated the performance of three commercially available dengue nonstructural 1 ns1 antigen assays biorad platelia dengue ns1 antigen elisa, panbio dengue early elisa, and biorad dengue ns1 antigen strip test and compared them. The dengue virus igm elisa is intended for the qualitative determination of igm class antibodies against dengue virus in human serum or plasma citrate, heparin. The dengue dxselect igm capture and igg elisa kits are designed for optimal performance and cost efficiency. Dengue igm capture elisa kit deia510 creative diagnostics. Not for sale or distribution in the united states of america. The test improvement resulted in a highly sensitive and specific test suitable for use as a firstline test in the field. Polystyrene microtiter wells are precoated with polyclonal capture antibodies. Comparison of two commercial elisa kits for the detection. Inform your doctor if you are on any medications or have any underlying medical conditions or allergies before undergoing dengue antibodies igg elisa. Dengue virus elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples.
Igm specific antibody, if present, binds to the antigen. I use a capture elisa kit from panbio that works great in detecting human anti dengue igg. Dengue virus denv a mosquitoborne flavivirus, is a significant human pathogen of global importance. Dengue ns1 elisa for early detection of dengue virus denv, is an elisa assay system for the detection of ns1 antigen in human serum. Evaluation of a commercial dengue ns1 antigencapture. Paired sera from patients with dengue n20 and japanese. Dengue, an acute viral disease, is caused by any one of the four denv serotypes, denv1, 2, 3, and 4. Keywords acute dengue infection, ns1 immunochromatog raphy, mac elisa. Any of the routinely used tests like, viral nucleic acid detection by reverse transcription polymerase chain reaction rtpcr, dengue ns1 protein antigen or anti dengue igm igg antibodies, can be used to diagnose a case of dengue, however, igm capture elisa has always been the choice due to its cost effectiveness, availability and ease of. Macelisa test for the detection of dengue igm antibodies in human serumplasma dengue igm microlisa 1.